Description
The LUMICKS C-Trap Dymo400 is a four-trap optical-tweezers microscope with integrated 3-colour confocal-microscopy and 5-channel microfluidics. It can be used to manipulate biomolecules, applying and measuring forces with pN resolution while simultaneously fluorescently imaging the sample with 3-colour scanning confocal microscopy. It is commonly used to study DNA:protein interactions by tethering individual DNA molecules between optically-trapped beads and imaging fluorescently labelled protein interacting with the DNA in real-time at single-molecule resolution. It can also be used to perform force spectroscopy to measure mechanical properties of biomolecules such as DNA, RNA and proteins. In addition measurements of cell mechanics and phase separation can be performed.
It is very user friendly and intuitive to use, requiring minimal user training compared to traditional home-built optical-tweezer microscopes. Furthermore the control software can be used to automate experiments, reducing user input and increasing reproducibility.
Specification
Optical Traps:
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Four optical-traps
Confocal Imaging:
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Excitation Wavelengths: Blue – 488 nm, Green – 561 nm, Red – 638 nm
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Confocal Scanning Range: X = 50 um, Y = 35 um
Temperature Control Range:
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25 – 45°C
Microfluidics:
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5-Channel Microfluidics with automated control
