Perfusion system: Automate Scientific ValveLink 8.2 with BubbleStop8 Syringe heater

Electrical stimulation chamber with perfusion and thermal control

Perfusion chamber with electrical stimulation, mounted on SP8 2P photon confocal imaging system

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Detailed Description

The AutoMate Scientific Valvelink perfusion system allows real-time or pre-programmed liquid switching in perfusion, physiology, biophysics, electrochemistry, or general liquid-handling research applications. The BubbleStop™ Syringe Heater with integrated temperature sensor and feedback will ‘off-gas’ in the syringe reservoirs to prevent bubble formation in the perfused solutions.

8-channel general-purpose stimulus generator for current and voltage-driven electrical stimulation with integrated stimulus isolation unit for each output channel. Operating in Download and Streaming mode (continuous downstreaming of pulses from connected computer).

STG4008 (8-channel stimulus generator):
– 8 analogue voltage outputs: -8 V to +8 V @ ±20 mA (2 mm connector)
– 8 analogue current outputs: -1.6 mA to +1.6 mA @ 120 V (2 mm connector)
– 8 trigger (TTL) inputs (BNC connector)
– 8 trigger (TTL) outputs (BNC connector)
– Digital I/O-port (8 bit input, 8 bit output)
– Time resolution: 20 µs

Magnetic Imaging / recording chamber with removable electrodes and heating elements.

Uses/Applications

Electrical stimulation of cells
Imaging direct and indirect electrical stimulation of cells
Cell sensitivity to biologically relevant voltage/current/frequency
Cell-condutive material interactions

Bubblestop8 60 ml Syringe Heater capable of heater perfused solution from ambient to 50oC

Stimulus generator:
Operating temperature 10 °C to 50 °C
Output voltage –8 V to +8 V @ max. ± 20 mA, 1 mV resolution. Time lag between Sync Out and voltage output 15 (± 2) µs.
Output current –1.6 mA to +1.6 mA @ max.120 V compliance voltage (between +I and –I)
Output current resolution 200 nA Output current slope 600 µA / µs @ RL = 10 kW Rise time (10-90 %) current, 0 – 100 µA 1.4 µs @ RL = 10 kW Time lag between Sync Out and current output 15 (± 2) µs @ RL = 10 kW Resolution 14 bit Time resolution 20 µs

Freely programmable output signals, including rectangular, ramp, sinusoidal. Maximum frequency (rectangular waveform) 25 kHz. Memory capacity for stored waveforms
Minimum time length of trigger input (TTL) 40 µs Time lag between trigger in and Sync Out 70 (± 10) µs. Maximum trigger input frequency 10 kHz Interface and connectors.
Synchronization for 8 digital channels via coax cable Interface (connection to computer).