Photon Lines MicAO 3DSR (with MicAOv1.3 software) Solent Scienific 3-ORIO7 Incubation chamber

Adaptive optics for super-resolution

Stochastic Optical Reconstruction Microscope with Solent incubator.

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Detailed Description

The Stochastic Optical Reconstruction Microscope allows fluorescence imaging to be performed with 20 nm resolution in x/y and ~40 nm in z (using a MicAO 3DSR Adaptive Optics device). Samples can be maintained at room temperature in a hydrated environment (Solent incubator), so the apparatus finds many applications in biological physics (e.g. live cells) and soft-condensed matter physics (e.g. peptide aggregates). There are four solid state lasers available that can be used to illuminate a sample at 405, 488, 561 and 647 nm. Three dimensional STORM imaging is possible using ellipsoidal point spread function engineering with the Adaptive Optics device. A Photometrics Evolve 512 EMCCD and a Hamamatsu ORCA Flash v2 sCMOS are available for image detection. The apparatus can also be used for dynamic single molecule fluorescence experiments and a number of microfluidic platforms are available. A Total Internal Reflection Fluorescence (TIRF) modality is available using an Olympus UAPON 100 1.49NA OTIRF lens. The apparatus is built on an Olympus IX71 inverted microscope frame, with a PRIOR ProScan III sample stage and a MadCity Labs C-focus system to compensate for thermal drift in the z-plane. The laser light is delivered through a vibrated optical fibre to scramble any coherent speckle artefacts.


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