The Spectramax M5 is a microplate/cuvette reader.
It has accurate temperature control, microplate shaking and comprehensive data management with different detection modes:
UV-Visible Absorbance (Abs); Fluorescence Intensity (FI); Luminescence (Lum); Time-Resolved Fluorescence (TRF) and Fluorescence Polarization (FP)
The optical systems use two scanning monochromators so the user can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers. Capability to measure the depth (optical pathlength) of samples with no temperature dependency. Sensor automatically normalizes the well absorbance, eliminating the need for standard curves, and for compounds with known absorptive properties, enables users to calculate concentrations directly from absorbance.
Plate formats 6, 12, 24, 48, 96, 384 wells
Light source Xenon Flash Lamp (1 joule/flash)
Detectors 2 photomultiplier tubes (PMT)
Shaker time 0 to 999 seconds
Temp. control 2°C above ambient to 60°C
Temp. uniformity < 1°C at 37°C set point Temp. accuracy ±1°C at 37°C set point Standard Read Times(minutes:seconds)* 96 wells 384 wells Absorbance 0:18 0:49 Fluorescence Intensity 0:17 0:48 Fluorescence Polarization 0:42 2:03 Time-Resolved Fluorescence 0:17 0:48 Luminescence 2:00 7:00
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